For ages, gene editing had a need for engineering DNA-cutting enzymes, named as zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs), which were required for every specific genetic alteration. The arrival of CRISPR-Cas9, a genome-editing tool that can work only with tailored guide RNA molecules that locates its target, transformed the field completely. An entire new age of the genome editing techniques, defined by regularly clustered interspaced short palindromic repeats/CRISPR-associated endonuclease 9 (CRISPR/Cas 9), is indeed an absolutely incredible technology developed by "Doudna and Charpentier" contributing to modern life sciences in the past decades, thus can not only bring predictable but inheritable changes too given at particular locations of the provided plant genome. That incorporates deletion then insertion followed by replacement in the base sequence in order to achieve accurate, error-free and specific Advancement of desired plant characters.