Several novel methodologies, including tracer studies, genomics, proteomics, systems modelling, molecular genetics, among others, have contributed to significant progress in fish larval nutrition in recent years and some techniques are already incorporated into the present by slight modifications in the system and some might have future application for the larval nutrition. Under these, the tube-feeding and incorporation of 14C-amino acids and lipids into artemia, Diet selection of fish larvae by different natural stable isotope signatures or diets with different rare metal oxides by using Mechanistic modelling. Population genomics can be used in assessing genotype effects on nutritional requirements of fish larvae, by using progeny testing in fish reared in the same tanks, and also in identifying QTLs for larval stages.