Crop improvement for any character depends upon the genetic variability present in that population. Mutation is the ultimate source of genetic variation in populations. Mutation can be utilized by researchers to improve both qualitative and quantitative characters. Mutagenesis can be done by giving a physical or chemical mutagen treatment. Along with these treatments, site-specific mutations can be used in mutagenesis. Growing the mutant population followed by selection or screening for particular desired traits using visual, morphological, and molecular screening helps select the desirable mutants. Instead of its low desirable frequency, it can be used for the creation of genetic variability, which can be used in varietal development programmes.