Cod liver oil (CLO) is widely used to separate and purify PUFA because it is a rich source of various polyunsaturated fatty acids (PUFA), which are both economical and healthy. Cod liver oil contains vitamin A and D in addition to PUFA, and because of this, it is utilised more frequently than any other fish oil. It has numerous health advantages that have improved the way diseases are treated in people. It is frequently utilised as a supplement for newborns as well as in the development of drugs and therapies. However, using cod liver oil excessively can have certain negative consequences on people.